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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
29/03/2019 |
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Data da última atualização: |
21/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMARGO, L. S. de A.; MUNK, M.; SALES, J. N.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; VIANA, J. H. M. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MICHELE MUNK, UFJF; JOSE NELIO SALES, UFLA; SABINE WOHLRES-VIANA, UFJF; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
JBRA Assisted Reproduction, v. 23, n. 1, p. 7-14, 2019. |
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DOI: |
10.5935/1518-0557.20180084 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Luiz Sergio Almeida Camargo. |
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Conteúdo: |
Objective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool. MenosObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of p... Mostrar Tudo |
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Palavras-Chave: |
In vivo maturation; MRNA; Ovum pick-up. |
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Thesaurus Nal: |
Gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195183/1/jbra-23-01-0007.pdf
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Marc: |
LEADER 02498naa a2200253 a 4500
001 2107759
005 2022-06-21
008 2019 bl uuuu u00u1 u #d
024 7 $a10.5935/1518-0557.20180084$2DOI
100 1 $aCAMARGO, L. S. de A.
245 $aDifferential gene expression between in vivo and in vitro maturation$ba comparative study with bovine oocytes derived from the same donor pool.$h[electronic resource]
260 $c2019
500 $aNa publicação: Luiz Sergio Almeida Camargo.
520 $aObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool.
650 $aGene expression
653 $aIn vivo maturation
653 $aMRNA
653 $aOvum pick-up
700 1 $aMUNK, M.
700 1 $aSALES, J. N.
700 1 $aWOHLRES-VIANA, S.
700 1 $aQUINTAO, C. C. R.
700 1 $aVIANA, J. H. M.
773 $tJBRA Assisted Reproduction$gv. 23, n. 1, p. 7-14, 2019.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 44 | |
| 7. |  | WOHLRES-VIANA, S.; FERNANDES, L. E.; REIS, D. L. R.; MACHADO, M. A.; VIANA, J. H. M. Point mutations in the luteinizing hormone receptor MRNA of holstein (Bos taurus) granulosa cells - partial results. In: CONGRESSO BRASILEIRO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Brasília, DF: Sociedade Brasileira de Genética, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
|    |
| 9. | | CAMARGO, L. S. de A.; PALUDO, F.; PEREIRA, M. M.; WOHLRES-VIANA, S.; GIOSO, M. M.; CARVALHO, B. C. de; QUINTAO, C. C. R.; VIANA, J. H. M. Absence of Sperm Factors as in the Parthenogenesis Does Not Interfere on BovineEmbryo Sensitiveness to Heat Shock at Pre-Implantation Stage. Reproduction in Domestic Animals, v. 51, p. 3-9, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | BOITÉ, M. C.; CAMARGO, L. S. A.; GUIMARÃES, M. F. M.; SERAPIÃO, R. V.; WOHLRES-VIANA, S.; VIANA, J. H. M.; SÁ, W. F.; NOGUEIRA, L. A. G. Analysis of gene expression of in vitro produced bovine blastocyst cultured for 72 hours post-vitrification. In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 16., 2008, Budapest. Abstracts... Budapest, 2008. p. 186| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; GRAZIA, J. G. V.; CAMARGO, L. S. de A.; MACHADO, M. A.; VIANA, J. H. M. Could the differential expression of luteinizing hormone receptor isoforms explain the varaiability in superovulatory responses in cattle? In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 41., 2015, Versailles. Reproductive performance: at the crossroads of genetics and the environment: abstracts. Versailles: IETS, 2015. p. 261.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | CAMARGOS, A.; WOHLRES-VIANA, S.; COSTA, I. F.; CAMARGO, L. S. de A.; FERREIRA, J. C.; RAMOS, A. A.; OBA, E. Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 69, n. 4, p. 821-829, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | COSTA, F. Q.; PEREIRA, M. M.; WOHLRES VIANA, S.; SERAPIAO, R. V.; CARVALHO, B. C.; VIANA, J. H. M.; NOGUEIRA, L. A. G.; CAMARGO, L. S. de A. Effect of heat stress during oocyte maturation on gene expression of in vitro-fertilized and parthenogenetic bovine blastocysts. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | WOHLRES-VIANA, S.; BOITE, M. C.; PEREIRA, M. M.; SA, W. F. DE; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Effect of production system and breed on relative expression in bovine embryos In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT, 21., 2009, San Diego. Proceeding... San Diego: Csiro, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | CAMARGO, L. S. de A.; MUNK, M.; SALES, J. N.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; VIANA, J. H. M. Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool. JBRA Assisted Reproduction, v. 23, n. 1, p. 7-14, 2019. Na publicação: Luiz Sergio Almeida Camargo.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | WOHLRES VIANA, S.; PEREIRA, M. M.; OLIVEIRA, A. P.; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Differences between Bos taurus and Bos indicus embryos: transcripts amounts. Reproduction, Fertility and Development, v. 22, n. 1, 2010. Edição dos proceedings do Annual Conference of the International Embryo, 2010, Cordoba.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 44 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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